Microbial gas generation potential and metabolic mechanism of in situ microorganisms in Jimsar area of southern Junggar Basin

XU Fan; WANG Gang; LI Yueguo; YANG Shuguang; YAN Detian; WANG Yibing; SU Xianbo; LAI Peng; WANG Bo; FU Haijiao

doi:10.19509/j.cnki.dzkq.tb20250382

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XU Fan，WANG Gang，LI Yueguo，et al. Microbial gas generation potential and metabolic mechanism of in situ microorganisms in Jimsar area of southern Junggar Basin[J]. Bulletin of Geological Science and Technology，2026，45（6）：1-11 doi: 10.19509/j.cnki.dzkq.tb20250382

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Microbial gas generation potential and metabolic mechanism of in situ microorganisms in Jimsar area of southern Junggar Basin

doi: 10.19509/j.cnki.dzkq.tb20250382

XU Fan^1
,,
WANG Gang^{1, 2},
LI Yueguo¹,
YANG Shuguang²,
YAN Detian¹,
WANG Yibing³,
SU Xianbo^{1, 4},
LAI Peng³,
WANG Bo³,
FU Haijiao^{1
,
,}

School of Resources, China University of Geosciences (Wuhan), Wuhan 430074, China
Geological Research Institute of Xinjiang Uygur Autonomous Region, Urumqi 830000, China
Xinjiang Yaxin Coalbed Methane Investment and Development (Group) Co., Ltd., Urumqi 830000, China
School of Resources and Environment, Henan Polytechnic University, Jiaozuo Henan 454000, China

More Information

Author Bio:
E-mail：1041992471@qq.com
Corresponding author: E-mail：fuhj@cug.edu.cn
Received Date: 20 Aug 2025
Accepted Date: 09 Nov 2025
Rev Recd Date: 20 Oct 2025

Available Online: 18 Nov 2025

Abstract

Abstract

Objective
The Jimsar area in the southern margin of the Junggar Basin is rich in low- to medium-rank coalbed methane (CBM) resources, showing distinct characteristics of microbial gas. To date, it remains unclear whether there is an active supply of microbial gas in the Jimsar area, which restricts the evaluation of CBM resource potential and the subsequent exploration deployment in this area.
Methods
In this study, the water samples of CBM development wells and the coal samples of adjacent CBM parameter wells in the Jimsar area were taken as the main research objects, and the actual coal reservoir medium environment was taken as the constraint condition. An anaerobic fermentation gas production simulation device under near in situ conditions was constructed. Based on the comparative analysis of gas production characteristics, changes in pore volume and pore size of coal samples, microbial community structure evolution, and microbial gene function characteristics at different stages, the gas production potential and metabolic mechanism of in situ microorganisms in the Jimsar area were investigated.
Results
The results showed that the in situ microorganisms in the Jimsar area had the potential to produce microbial gas under the current conditions, and the cumulative gas production of CH₄ in the experiment was 9.49 × 10⁻² mL/g. Metagenomic sequencing revealed that there were many types and high abundances of hydrolytic bacteria in the in situ microorganisms of the Jimsar area. The typical acidogenic fermentation bacteria and hydrogen-producing acetogenic bacteria were relatively scarce, and the methanogenic archaea were mainly Methanosarcina. Although there were relatively few acidogenic fermentation bacteria and hydrogen-producing acetogenic bacteria, most of the hydrolytic bacteria could directly degrade the macromolecules in coal into short-chain fatty acids, acetic acid, hydrogen and carbon dioxide, which could provide sufficient substrates for methanogenic archaea.
Conclusion
In the early stage of anaerobic fermentation, Aliarcobacter rapidly proliferates and inhibits hydrolytic bacteria such as Pseudomonas. The abundance of gene functions such as carbohydrate metabolism decreases, and the hydrolysis process is limited, which cannot provide sufficient substrates for methanogens. The abundance of the methanogen Methanosarcina decreases, and the amount of methane produced in the stage decreases. Subsequently, the reduction in Aliarcobacter abundance is followed by the reactivation of hydrolytic bacteria and an enhancement in the functional potential for carbohydrate metabolism and xenobiotics biodegradation. This leads to the enrichment of substrates available for methanogens, which results in an increased abundance of Methanosarcina and a concurrent rise in methane production. The findings can provide a theoretical basis for the evaluation of low- to medium-rank CBM resources, the formulation of exploration plans, and the smooth implementation of coalbed gas bioengineering.
- in situ microorganisms,
- coalbed methane,
- anaerobic fermentation,
- metabolic mechanism,
- near in situ condition,
- Jimsar,
- Junggar Basin

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References

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Microbial gas generation potential and metabolic mechanism of in situ microorganisms in Jimsar area of southern Junggar Basin

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Microbial gas generation potential and metabolic mechanism of in situ microorganisms in Jimsar area of southern Junggar Basin

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